![]() |
²úµØ | ÉϺ£ |
±£´æÌõ¼þ | 2-8¡æ |
Æ·ÅÆ | DM |
»õºÅ | DM5964 |
ÓÃ; | ¿ÆÑÐÓëʵÑé |
¼ì²â·½·¨ | elisa |
±£ÖÊÆÚ | 6¸öÔÂ |
ÊÊÓ¦ÎïÖÖ | ´óÊó |
¼ì²âÏÞ | µçѯ |
ÊýÁ¿ | ‰äÁ¿ÏÖ»õ |
°ü×°¹æ¸ñ | 96T/48T |
±ê¼ÇÎï | µçѯ |
Ñù±¾ | ÌåÒº¡¢ÑªÇ塢Ѫ½¬¡¢Ï¸°ûÅàÑøÉÏÇåÒº¡¢ÄòÒº¡¢×éÖ¯ÔȽ¬µÈ |
Ó¦Óà | µçѯ |
ÊÇ·ñ½ø¿Ú | ·ñ |
ÈËÊӻƴ¼½áºÏµ°°×1(RBP1)øÁªÃâÒßÊÔ¼ÁºÐ
²úÆ·»õºÅ£ºDM5964
ÉϺ£óÆÂêÉúÎï¿Æ¼¼ÓÐÏÞ¹«Ë¾¹©Ó¦µÄ¾¹ú¼ÒÈÏÖ¤£¬ÒÔÃâÒßѧ·´Ó¦Îª»ù´¡£¬ÔÚͬµÈÖÊÁ¿Ìõ¼þÏÂͨ¹ý´ó¹æÄ£Éú²ú»ò¼¼Êõ½ø²½½µµÍ³É±¾£¬²¢µÃµ½ µÄÈ·Ö¤·½·¨ºÍÈ·Ö¤µÄÑùÆ·½øÐмì²â¡£¹«Ë¾×¨ÒµÌṩ¸÷ÖÖÖÖÊô£¬¹©Ó¦µÄELISAÊÔ¼ÁºÐͳһ¼ÛÏúÊÛ¡£
²úÆ·Ãû³Æ£ºÈËÊӻƴ¼½áºÏµ°°×1(RBP1)øÁªÃâÒßÊÔ¼ÁºÐ
±£´æÎȶ¨£º2-8¡æ
¼ì²âÔÀí£º²ÉÓÃË«¿¹Ìå¼ÐÐÄABC-ELISAÔÀí
Ö÷ÒªÓÃ;£º¿ÆÑмì²â£¬²»µÃÓÃÓÚÁÙ´²Õï¶Ï¡£
ELISAÊÔ¼ÁºÐ¼ì²â·¶Î§£º
ÈË¡¢ÃàÑò¡¢Ð¡Êó¡¢´óÊó¡¢Öí¡¢Íá¢É½Ñò¡¢Å£¡¢Âí¡¢Öí¡¢ÆäËü¶¯Îïϸ°ûÒò×Ó¡¢Ö²Îïϸ°ûÒò×Ó¡¢¹Ç´úл¡¢Ï¸°ûµòÍö¡¢¼¤ËØ¡¢»îÐÔ¶àëÄ¡¢×ÔÉí¿¹Ì塢ֹѪ¡¢×ÔÉí¿¹Ìå¿ÆÑÐElisa¼ì²âÊÔ¼ÁºÐ¡£
ÈËÌÇÁ´¿¹Ô50(ca50)ELISAÊÔ¼ÁºÐELISAÊÔ¼ÁºÐ¼ì²âÀàÐÍ£ºË«¿¹Ìå¼ÐÐÄ·¨²â¿¹Ô¡¢Ë«¿¹Ô¼ÐÐÄ·¨²â¿¹Ìå¡¢¼ä½Ó·¨²â¿¹Ìå¡¢¾ºÕù·¨²â¿¹Ìå¡¢¾ºÕù·¨²â¿¹Ô¡¢²¶»ñ°ü±»·¨²â¿¹Ìå¡¢ABS-ELISA·¨¡£
ÿ´ÎʵÑéµÄ±ê×¼ÇúÏßÒ»°ãÉè7¸ö²»Í¬Å¨¶È£¬¼ÓÉϿհ׿ף¬±ê×¼ÇúÏßÒ»¹²ÐèÒª8¸ö¿×¡£Òò´Ë£¬Ò»¸ö48TÊÔ¼ÁºÐ×î¶à¿ÉÒԲⶨ40¸öÑù±¾£¨²»×öÖØ¸´ÇÒÒ»´ÎÓÃÍ꣩£¬Ò»¸ö96TÊÔ¼ÁºÐ×î¶à¿ÉÒԲⶨ88¸öÑù±¾£¨²»×öÖØ¸´ÇÒÒ»´ÎÓÃÍ꣩¡£¿ÉÒÔ¸ù¾Ýʵ¼ÊÑù±¾ÊýÁ¿ºÍʵÑ鼯»®Ñ¡ÔñºÏÊʵIJúÆ·¹æ¸ñ¡£
ÈËÌÇÁ´¿¹Ô50(ca50)ELISAÊÔ¼ÁºÐÊÔ¼ÁµÄ×¼±¸£º
°´ÊÔ¼ÁºÐ˵Ã÷ÊéµÄÒªÇó×¼±¸ÊµÑéÖÐÐèÓõÄÊÔ¼Á¡£ELISAÖÐÓõÄÕôÁóË®»òÈ¥Àë×ÓË®£¬°üÀ¨ÓÃÓÚÏ´µÓµÄ£¬Ó¦ÎªÐÂÏʵĺ͸ßÖÊÁ¿µÄ¡£×ÔÅäµÄ»º³åÒºÓ¦ÓÃpH¼Æ²âÁ¿½ÏÕý¡£´Ó±ùÏäÖÐÈ¡³öµÄÊÔÑéÓÃÊÔ¼ÁÓ¦´ýζÈÓëÊÒÎÂÆ½ºâºóʹÓá£ÊÔ¼ÁºÐÖб¾´ÎÊÔÑé²»ÐèÓõIJ¿·ÖÓ¦¼°Ê±·Å»Ø±ùÏä±£´æ¡£
ÊÔ¼ÁºÐ×é³É¼°ÊÔ¼ÁÅäÖÆ
1¡¢ øÁª°å£¨Assay plate £©£ºÒ»¿é£¨96¿×»ò48¿×£©¡£
2¡¢±ê׼Ʒ£¨Standard£©£º2Æ¿£¨¶³¸ÉÆ·£©¡£
3¡¢ÑùÆ·Ï¡ÊÍÒº£¨Sample Diluent£©£º1¡Á20ml/Æ¿¡£
4¡¢ÉúÎïËØ±ê¼Ç¿¹ÌåÏ¡ÊÍÒº£¨Biotin-antibody Diluent£©£º1¡Á10ml/Æ¿¡£
5¡¢ À±¸ù¹ýÑõ»¯Îïø±ê¼ÇÇ׺ÍËØÏ¡ÊÍÒº £¨HRP-avidin Diluent£©£º1¡Á10ml/Æ¿¡£
6¡¢ÉúÎïËØ±ê¼Ç¿¹Ì壨Biotin-antibody£©£º1¡Á120¦Ìl/Æ¿£¨1£º100£©
7¡¢ À±¸ù¹ýÑõ»¯Îïø±ê¼ÇÇ׺ÍËØ£¨HRP-avidin£©£º1¡Á120¦Ìl/Æ¿£¨1£º100£©
8¡¢ µ×ÎïÈÜÒº£¨TMB Substrate£©£º1¡Á10ml/Æ¿¡£
9¡¢ ŨϴµÓÒº£¨Wash Buffer£©£º1¡Á20ml/Æ¿£¬Ê¹ÓÃʱÿƿÓÃÕôÁóˮϡÊÍ25±¶¡£
10¡¢ ÖÕÖ¹Òº£¨Stop Solution£©£º1¡Á10ml/Æ¿£¨2N H2SO4£©¡£
²Ù×÷²½Öè
ÈËÊӻƴ¼½áºÏµ°°×1(RBP1)øÁªÃâÒßÊÔ¼ÁºÐ
¼ì²â½á¹û¼ÆË㣺
·½·¨Ò»£º ÒÔ±ê×¼ÎïµÄŨ¶ÈΪºá×ø±ê£¬ODֵΪ×Ý×ø±ê£¬ÔÚ×ø±êÖ½ÉÏ»æ³ö±ê×¼ÇúÏߣ¬¸ù¾ÝÑùÆ·µÄODÖµÓɱê×¼ÇúÏß²é³öÏàÓ¦µÄŨ¶È£»ÔÙ³ËÒÔÏ¡Êͱ¶Êý£»
·½·¨¶þ£ºÓñê×¼ÎïµÄŨ¶ÈÓëODÖµ¼ÆËã³ö±ê×¼ÇúÏßµÄÖ±Ï߻ع鷽³Ìʽ£¬½«ÑùÆ·µÄODÖµ´úÈë·½³Ìʽ£¬¼ÆËã³öÑùƷŨ¶È£¬ÔÙ³ËÒÔÏ¡Êͱ¶Êý£¬¼´ÎªÑùÆ·µÄʵ¼ÊŨ¶È¡£
ÉϺ£óÆÂêÉúÎï¿Æ¼¼ÓÐÏÞ¹«Ë¾