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    Histone H3(mono methyl K36) [SR04-20]
    • 品牌:OminimAbs
    • 产地:上海
    • 型号:毫升
    • 货号:OM641708
    • 发布日期: 2024-09-14
    • 更新日期: 2024-10-09
    产品详请
    产地 上海
    品牌 OminimAbs
    保存条件
    货号 OM641708
    用途 实验研究
    应用范围 科研实验
    抗原来源 电询
    保质期 2年
    抗体名 电询
    是否单克隆
    克隆性
    靶点 电询
    适应物种 电询
    形态 电询
    宿主 电询
    标记物 电询
    包装规格 毫升
    亚型 电询
    标识物 电询
    浓度 电询%
    免疫原 电询
    是否进口

    Product Profile

    Product Name

    Histone H3(mono methyl K36) [SR04-20]

    Antibody Type

    Primary Antibodies

    Product description

    In eukaryotes, DNA is wrapped around histone octamers to form the basic unit of chromatin structure. The octamer is composed of histones H2A, H2B, H3 and H4, and it associates with approximately 200 base pairs of DNA to form the nucleosome. The association of DNA with histones results in dense packing of chromatin, which restricts proteins involved in gene transcription from binding to DNA. Histone H3, the core protein of the nucleosome, becomes phosphorylated at the end of prophase. The two major sites of phosphorylation are the mitosis-specific site Ser10, and Ser28, both of which are extensively phosphorylated in DNA-bound forms of histone H3 and in nucleosomal histone H3. The nucleosome structure of histone H3 promotes N-terminal phosphorylation in vitro.

    More 

    Immunogen

    recombinant protein

    Key Feature

    Clonality

    Monoclonal

    Isotype

    IgG

    Host Species

    Recombinant rabbit

    Tested Applications

    WBICC/IF

    WB:1:1,000-1:2,000
    ICC:1:50-1:200

    Species Reactivity

    HumanMouse

    Concentration

    1 mg/mL.

    Target Information

    Alternative Names

    Molecular Weight(MW)

    15 kDa

    Cellular Localization

    Nucleus, Chromosome.

    Database Links

    SwissProt ID

    P68431
    P84243
    Q16695
    Q6NXT2
    Q71DI3
    P84244

    Application

    ·        

    Application

    Fig1: Western blot analysis of Histone H3(mono methyl K36) on different lysates using anti-Histone H3(mono methyl K36) antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: NIH/3T3

    ·        

    Application

    Fig2: ICC staining Histone H3(mono methyl K36) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Positive Control

    NIH/3T3, MCF-7, Hela.

    Application Notes

    WB:1:1,000-1:2,000
    ICC:1:50-1:200

    Additional Information

    Form

    Liquid

    Storage Instructions

    Store at +4 after thawing. Aliquot store at -20 or -80. Avoid repeated freeze / thaw cycles.

    Storage Buffer

    1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.


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