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Product Profile

Product Name	Phospho-Creb (Ser 133)
Antibody Type	Primary Antibodies
Product description	Eukaryotic gene transcription is regulated by sequence-specific transcription factors that bind modular cis acting promoter and enhancer elements. The ATF/CREB transcription factor family binds the palindromic cAMP response element (CRE) octanucleotide TGACGTCA. The ATF/CREB family includes CREB-1, CREB-2 (also designated ATF-4), ATF-1, ATF-2 and ATF-3. This family of proteins contain highly divergent N-terminal domains, but share a C-terminal leucine zipper for dimerization and DNA binding. Although CREB can bind to DNA in an unphosphorylated state, it cannot activate transcription. Phosphorylation of CREB on Ser133 by protein kinase A facilitates its interaction with the CREB-binding protein (CBP) and activates the basal transcription complex. CREB functions in neoglucogenesis through interactions with the nuclear coactivator PGC-1. CREB may play a role in the pathogenesis of type II diabetes and dilated cardiomyopathy. The gene encoding CREB-1 maps to human chromosome 2q32.3-q34. Hide
Immunogen	A short amino acid sequence containing Ser 133 phosphorylated CREB-1 of human origin.

Key Feature

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Applications	WBIPIFIHC-P
	WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein (1 ml of cell lysate) IF:1:50-1:500 IHC:1:50-1:500 Hide
Species Reactivity	HumanMouseRat
Concentration	1 mg/mL.

Target Information

Alternative Names	Active transcription factor CREB antibody cAMP response element binding protein 1 antibody cAMP response element binding protein antibody cAMP responsive element binding protein 1 antibody cAMP-responsive element-binding protein 1 antibody CREB antibody CREB-1 antibody CREB1 antibody CREB1_HUMAN antibody Cyclic AMP-responsive element-binding protein 1 antibody MGC9284 antibody OTTHUMP00000163864 antibody OTTHUMP00000163865 antibody OTTHUMP00000206660 antibody OTTHUMP00000206662 antibody OTTHUMP00000206667 antibody Transactivator protein antibody Hide
Molecular Weight(MW)	43kDa
Cellular Localization	Nucleus

Database Links

SwissProt ID

P16220

Application

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Application

Fig1: Western blot analysis of CREB-1 phosphorylation in non-transfected (A,D), untreatedmo use CREB-1 transfected (B,E) and lambda protein phosphatase treatedmouse CREB-1 transfected (C,F) 293T whole cell lysates. Antibodies tested include p-CREB-1 (Ser 133)(A,B,C) andCREB- 1 (C-21)(D,E,F).

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Application

Fig2： Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear staining.

Positive Control	293T, human breast carcinoma tissue.
Application Notes	WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein (1 ml of cell lysate) IF:1:50-1:500 IHC:1:50-1:500 Hide

Additional Information

Form	Liquid
Storage Instructions	Store at +4℃
Storage Buffer	1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.