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Product Profile

Product Name	CREB (phospho Ser129) antibody
Antibody Type	Primary Antibodies
Product descriptio	Rabbit Polyclonal antibody to CREB (Phospho Ser129)This protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Implicated in synchronization of circadian rhythmicity. Hide
Immunogen	Peptide sequence around phosphorylation site of serine 129 (I-L-S(p)-R-R) derived from Human CREB.

Key Feature

Clonality	Polyclonal
Host Species	Rabbit
Tested Application	IHC-PWB
	Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: . Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.:
Species Reactivity	HumanMouseRat
Concentration	1 mg/ml (Please refer to the vial label for the specific concentration)
Purification	Affinity purified

Target Information

Gene Synonyms	CREB MGC9284 CREB1 cyclic AMP-responsive element-binding protein 1 active transcription factor CREB transactivator protein CREB-1 cAMP-response element-binding protein-1 cAMP-responsive element-binding protein 1 cAMP responsive element binding pr Hide
Target	CREB (Phospho Ser129)
Molecular Weight(MW)	43 (note)

Application

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Application

Western blot analysis of extracts from 293 cells untreated (lane 1) or treated with UV (lane 2) using CREB (Phospho-Ser129) Antibody .

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Application

Western blot analysis of extracts from 293 cells untreated (lane 1) or treated with UV (lane 2) using CREB (Phospho-Ser129) Antibody

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Application

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CREB (Phospho-Ser129) Antibody (left) or the same antibody preincubated with blocking peptide (right).

Application Notes

Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: . Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.:

Additional Information

Form	Liquid
Storage Instruction	Keep as concentrated solution. Aliquot and store at -20oC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer	saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.