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Product Profile

Product Name	Anti-ITK antibody
Antibody Type	Primary Antibodies
Immunogen	Polypeptide

Key Feature

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Application	ELISAICC/IFIHCWB
	WB:1:200-1:2000 IHC:1:200-1:2000 ICC/IF:1:100-1:500
Species Reactivity	HumanMouseRat
Concentration	1mg/ml
Purification	Protein A

Target Information

Gene Symbol	ITK
Gene Synonyms	EMT LYK LPFS1 PSCTK2 Hide
Gene Full Name	IL2 inducible T cell kinase
Gene Summary	This gene encodes an intracellular tyrosine kinase expressed in T-cells. The protein contains both SH2 and SH3 domains which are often found in intracellular kinases. It is thought to play a role in T-cell proliferation and differentiation. [provided by RefSeq, Jul 2008]
Molecular Weight(MW)	72KD
Cellular Localizatio	Cytoplasm, Nucleus

Application

·

WB

Western blot analysis using ITK antibody against HepG2(1) cell lysate.

·

IHC

Immunohistochemical analysis of paraffin-embedded breast cancer tissues using ITK antibody with DAB staining.

·

ICC/IF

Immunofluorescence analysis of A549 cells using ITK antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Application Notes

WB:1:200-1:2000 IHC:1:200-1:2000 ICC/IF:1:100-1:500

Additional Information

Form	Liquid
Storage Instruction	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer	Purified antibody in PBS with 0.05% sodium azide.

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