|
| 产地 | 上海 |
| 品牌 | OminimAbs |
| 保存条件 | |
| 货号 | OM641708 |
| 用途 | 实验研究 |
| 应用范围 | 科研实验 |
| 抗原来源 | 电询 |
| 保质期 | 2年 |
| 抗体名 | 电询 |
| 是否单克隆 | 否 |
| 克隆性 | 否 |
| 靶点 | 电询 |
| 适应物种 | 电询 |
| 形态 | 电询 |
| 宿主 | 电询 |
| 标记物 | 电询 |
| 包装规格 | 毫升 |
| 亚型 | 电询 |
| 标识物 | 电询 |
| 浓度 | 电询% |
| 免疫原 | 电询 |
| 是否进口 | 否 |
Product Profile
Product Name
Anti-CDC27 antibody
Antibody Type
Primary Antibodies
Immunogen
Polypeptide
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested
Application
ELISAICC/IFIHCWB
WB:1:200-1:2000
IHC:1:200-1:1000
ICC/IF:1:100-1:500
Species
Reactivity
HumanMouseRat
Concentration
1 mg/ml
Purification
Protein A
Target Information
Gene Symbol
CDC27
Gene Synonyms
APC3
HNUC
NUC2
H-NUC
ANAPC3
CDC27Hs
D0S1430E
D17S978E
Hide
Gene Full Name
cell division cycle 27
Gene Summary
The protein encoded by this gene shares
strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene
product of Schizosaccharomyces pombe nuc 2. This protein is a component of
the anaphase-promoting complex (APC), which is composed of eight protein
subunits and is highly conserved in eukaryotic cells. This complex catalyzes
the formation of cyclin B-ubiquitin conjugate, which is responsible for the
ubiquitin-mediated proteolysis of B-type cyclins. The protein encoded by this
gene and three other members of the APC complex contain tetratricopeptide
(TPR) repeats, which are important for protein-protein interactions. This
protein was shown to interact with mitotic checkpoint proteins including
Mad2, p55CDC and BUBR1, and it may thus be involved in controlling the timing
of mitosis. Alternative splicing of this gene results in multiple transcript
variants. Related pseudogenes have been identified on chromosomes 2, 22 and
Y.
Hide
Molecular
Weight(MW)
92 kDa
Cellular
Localizatio
Cytoplasm, Cytoskeleton, Nucleus
Application
·
WB
Western blot
analysis using CDC27 antibody against HEK293(1) cell lysate.
·
IHC
Immunohistochemical
analysis of paraffin-embedded bladder cancer tissues using CDC27 antibody with
DAB staining.
·
ICC/IF
Immunofluorescence
analysis of CAL-27 cells using CDC27 antibody (green). Blue: DAPI fluorescent
DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555
phalloidin.
Application
Notes
WB:1:200-1:2000
IHC:1:200-1:1000
ICC/IF:1:100-1:500
Additional Information
Form
Liquid
Storage
Instruction
Shipped at 4°C. Store at +4°C short term
(1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer
Purified antibody in PBS with 0.05%
sodium azide.
Product Profile
Product Name
Anti-CDC27 antibody
Antibody Type
Primary Antibodies
Immunogen
Polypeptide
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested
Application
ELISAICC/IFIHCWB
WB:1:200-1:2000
IHC:1:200-1:1000
ICC/IF:1:100-1:500
Species
Reactivity
HumanMouseRat
Concentration
1 mg/ml
Purification
Protein A
Target Information
Gene Symbol
CDC27
Gene Synonyms
APC3
HNUC
NUC2
H-NUC
ANAPC3
CDC27Hs
D0S1430E
D17S978E
Hide
Gene Full Name
cell division cycle 27
Gene Summary
The protein encoded by this gene shares
strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene
product of Schizosaccharomyces pombe nuc 2. This protein is a component of
the anaphase-promoting complex (APC), which is composed of eight protein
subunits and is highly conserved in eukaryotic cells. This complex catalyzes
the formation of cyclin B-ubiquitin conjugate, which is responsible for the
ubiquitin-mediated proteolysis of B-type cyclins. The protein encoded by this
gene and three other members of the APC complex contain tetratricopeptide
(TPR) repeats, which are important for protein-protein interactions. This
protein was shown to interact with mitotic checkpoint proteins including
Mad2, p55CDC and BUBR1, and it may thus be involved in controlling the timing
of mitosis. Alternative splicing of this gene results in multiple transcript
variants. Related pseudogenes have been identified on chromosomes 2, 22 and
Y.
Hide
Molecular
Weight(MW)
92 kDa
Cellular
Localizatio
Cytoplasm, Cytoskeleton, Nucleus
Application
·
WB
Western blot
analysis using CDC27 antibody against HEK293(1) cell lysate.
·
IHC
Immunohistochemical
analysis of paraffin-embedded bladder cancer tissues using CDC27 antibody with
DAB staining.
·
ICC/IF
Immunofluorescence
analysis of CAL-27 cells using CDC27 antibody (green). Blue: DAPI fluorescent
DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555
phalloidin.
Application
Notes
WB:1:200-1:2000
IHC:1:200-1:1000
ICC/IF:1:100-1:500
Additional Information
Form
Liquid
Storage
Instruction
Shipped at 4°C. Store at +4°C short term
(1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer
Purified antibody in PBS with 0.05%
sodium azide.
Product Profile
Product Name
Anti-CDC27 antibody
Antibody Type
Primary Antibodies
Immunogen
Polypeptide
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested
Application
ELISAICC/IFIHCWB
WB:1:200-1:2000
IHC:1:200-1:1000
ICC/IF:1:100-1:500
Species
Reactivity
HumanMouseRat
Concentration
1 mg/ml
Purification
Protein A
Target Information
Gene Symbol
CDC27
Gene Synonyms
APC3
HNUC
NUC2
H-NUC
ANAPC3
CDC27Hs
D0S1430E
D17S978E
Hide
Gene Full Name
cell division cycle 27
Gene Summary
The protein encoded by this gene shares
strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene
product of Schizosaccharomyces pombe nuc 2. This protein is a component of
the anaphase-promoting complex (APC), which is composed of eight protein
subunits and is highly conserved in eukaryotic cells. This complex catalyzes
the formation of cyclin B-ubiquitin conjugate, which is responsible for the
ubiquitin-mediated proteolysis of B-type cyclins. The protein encoded by this
gene and three other members of the APC complex contain tetratricopeptide
(TPR) repeats, which are important for protein-protein interactions. This
protein was shown to interact with mitotic checkpoint proteins including
Mad2, p55CDC and BUBR1, and it may thus be involved in controlling the timing
of mitosis. Alternative splicing of this gene results in multiple transcript
variants. Related pseudogenes have been identified on chromosomes 2, 22 and
Y.
Hide
Molecular
Weight(MW)
92 kDa
Cellular
Localizatio
Cytoplasm, Cytoskeleton, Nucleus
Application
·
WB
Western blot
analysis using CDC27 antibody against HEK293(1) cell lysate.
·
IHC
Immunohistochemical
analysis of paraffin-embedded bladder cancer tissues using CDC27 antibody with
DAB staining.
·
ICC/IF
Immunofluorescence
analysis of CAL-27 cells using CDC27 antibody (green). Blue: DAPI fluorescent
DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555
phalloidin.
Application
Notes
WB:1:200-1:2000
IHC:1:200-1:1000
ICC/IF:1:100-1:500
Additional Information
Form
Liquid
Storage
Instruction
Shipped at 4°C. Store at +4°C short term
(1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer
Purified antibody in PBS with 0.05%
sodium azide.
Product Profile
Product Name
Anti-CDC27 antibody
Antibody Type
Primary Antibodies
Immunogen
Polypeptide
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested
Application
ELISAICC/IFIHCWB
WB:1:200-1:2000
IHC:1:200-1:1000
ICC/IF:1:100-1:500
Species
Reactivity
HumanMouseRat
Concentration
1 mg/ml
Purification
Protein A
Target Information
Gene Symbol
CDC27
Gene Synonyms
APC3
HNUC
NUC2
H-NUC
ANAPC3
CDC27Hs
D0S1430E
D17S978E
Hide
Gene Full Name
cell division cycle 27
Gene Summary
The protein encoded by this gene shares
strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene
product of Schizosaccharomyces pombe nuc 2. This protein is a component of
the anaphase-promoting complex (APC), which is composed of eight protein
subunits and is highly conserved in eukaryotic cells. This complex catalyzes
the formation of cyclin B-ubiquitin conjugate, which is responsible for the
ubiquitin-mediated proteolysis of B-type cyclins. The protein encoded by this
gene and three other members of the APC complex contain tetratricopeptide
(TPR) repeats, which are important for protein-protein interactions. This
protein was shown to interact with mitotic checkpoint proteins including
Mad2, p55CDC and BUBR1, and it may thus be involved in controlling the timing
of mitosis. Alternative splicing of this gene results in multiple transcript
variants. Related pseudogenes have been identified on chromosomes 2, 22 and
Y.
Hide
Molecular
Weight(MW)
92 kDa
Cellular
Localizatio
Cytoplasm, Cytoskeleton, Nucleus
Application
·
WB
Western blot
analysis using CDC27 antibody against HEK293(1) cell lysate.
·
IHC
Immunohistochemical
analysis of paraffin-embedded bladder cancer tissues using CDC27 antibody with
DAB staining.
·
ICC/IF
Immunofluorescence
analysis of CAL-27 cells using CDC27 antibody (green). Blue: DAPI fluorescent
DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555
phalloidin.
Application
Notes
WB:1:200-1:2000
IHC:1:200-1:1000
ICC/IF:1:100-1:500
Additional Information
Form
Liquid
Storage
Instruction
Shipped at 4°C. Store at +4°C short term
(1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer
Purified antibody in PBS with 0.05%
sodium azide.
Product Profile
Product Name
Anti-CDC27 antibody
Antibody Type
Primary Antibodies
Immunogen
Polypeptide
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested
Application
ELISAICC/IFIHCWB
WB:1:200-1:2000
IHC:1:200-1:1000
ICC/IF:1:100-1:500
Species
Reactivity
HumanMouseRat
Concentration
1 mg/ml
Purification
Protein A
Target Information
Gene Symbol
CDC27
Gene Synonyms
APC3
HNUC
NUC2
H-NUC
ANAPC3
CDC27Hs
D0S1430E
D17S978E
Hide
Gene Full Name
cell division cycle 27
Gene Summary
The protein encoded by this gene shares
strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene
product of Schizosaccharomyces pombe nuc 2. This protein is a component of
the anaphase-promoting complex (APC), which is composed of eight protein
subunits and is highly conserved in eukaryotic cells. This complex catalyzes
the formation of cyclin B-ubiquitin conjugate, which is responsible for the
ubiquitin-mediated proteolysis of B-type cyclins. The protein encoded by this
gene and three other members of the APC complex contain tetratricopeptide
(TPR) repeats, which are important for protein-protein interactions. This
protein was shown to interact with mitotic checkpoint proteins including
Mad2, p55CDC and BUBR1, and it may thus be involved in controlling the timing
of mitosis. Alternative splicing of this gene results in multiple transcript
variants. Related pseudogenes have been identified on chromosomes 2, 22 and
Y.
Hide
Molecular
Weight(MW)
92 kDa
Cellular
Localizatio
Cytoplasm, Cytoskeleton, Nucleus
Application
·
WB
Western blot
analysis using CDC27 antibody against HEK293(1) cell lysate.
·
IHC
Immunohistochemical
analysis of paraffin-embedded bladder cancer tissues using CDC27 antibody with
DAB staining.
·
ICC/IF
Immunofluorescence
analysis of CAL-27 cells using CDC27 antibody (green). Blue: DAPI fluorescent
DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555
phalloidin.
Application
Notes
WB:1:200-1:2000
IHC:1:200-1:1000
ICC/IF:1:100-1:500
Additional Information
Form
Liquid
Storage
Instruction
Shipped at 4°C. Store at +4°C short term
(1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer
Purified antibody in PBS with 0.05%
sodium azide.
Product Profile
Product Name
Anti-CDC27 antibody
Antibody Type
Primary Antibodies
Immunogen
Polypeptide
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested
Application
ELISAICC/IFIHCWB
WB:1:200-1:2000
IHC:1:200-1:1000
ICC/IF:1:100-1:500
Species
Reactivity
HumanMouseRat
Concentration
1 mg/ml
Purification
Protein A
Target Information
Gene Symbol
CDC27
Gene Synonyms
APC3
HNUC
NUC2
H-NUC
ANAPC3
CDC27Hs
D0S1430E
D17S978E
Hide
Gene Full Name
cell division cycle 27
Gene Summary
The protein encoded by this gene shares
strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene
product of Schizosaccharomyces pombe nuc 2. This protein is a component of
the anaphase-promoting complex (APC), which is composed of eight protein
subunits and is highly conserved in eukaryotic cells. This complex catalyzes
the formation of cyclin B-ubiquitin conjugate, which is responsible for the
ubiquitin-mediated proteolysis of B-type cyclins. The protein encoded by this
gene and three other members of the APC complex contain tetratricopeptide
(TPR) repeats, which are important for protein-protein interactions. This
protein was shown to interact with mitotic checkpoint proteins including
Mad2, p55CDC and BUBR1, and it may thus be involved in controlling the timing
of mitosis. Alternative splicing of this gene results in multiple transcript
variants. Related pseudogenes have been identified on chromosomes 2, 22 and
Y.
Hide
Molecular
Weight(MW)
92 kDa
Cellular
Localizatio
Cytoplasm, Cytoskeleton, Nucleus
Application
·
WB
Western blot
analysis using CDC27 antibody against HEK293(1) cell lysate.
·
IHC
Immunohistochemical
analysis of paraffin-embedded bladder cancer tissues using CDC27 antibody with
DAB staining.
·
ICC/IF
Immunofluorescence
analysis of CAL-27 cells using CDC27 antibody (green). Blue: DAPI fluorescent
DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555
phalloidin.
Application
Notes
WB:1:200-1:2000
IHC:1:200-1:1000
ICC/IF:1:100-1:500
Additional Information
Form
Liquid
Storage
Instruction
Shipped at 4°C. Store at +4°C short term
(1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer
Purified antibody in PBS with 0.05%
sodium azide.
Product Profile
Product Name
Anti-CDC27 antibody
Antibody Type
Primary Antibodies
Immunogen
Polypeptide
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested
Application
ELISAICC/IFIHCWB
WB:1:200-1:2000
IHC:1:200-1:1000
ICC/IF:1:100-1:500
Species
Reactivity
HumanMouseRat
Concentration
1 mg/ml
Purification
Protein A
Target Information
Gene Symbol
CDC27
Gene Synonyms
APC3
HNUC
NUC2
H-NUC
ANAPC3
CDC27Hs
D0S1430E
D17S978E
Hide
Gene Full Name
cell division cycle 27
Gene Summary
The protein encoded by this gene shares
strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene
product of Schizosaccharomyces pombe nuc 2. This protein is a component of
the anaphase-promoting complex (APC), which is composed of eight protein
subunits and is highly conserved in eukaryotic cells. This complex catalyzes
the formation of cyclin B-ubiquitin conjugate, which is responsible for the
ubiquitin-mediated proteolysis of B-type cyclins. The protein encoded by this
gene and three other members of the APC complex contain tetratricopeptide
(TPR) repeats, which are important for protein-protein interactions. This
protein was shown to interact with mitotic checkpoint proteins including
Mad2, p55CDC and BUBR1, and it may thus be involved in controlling the timing
of mitosis. Alternative splicing of this gene results in multiple transcript
variants. Related pseudogenes have been identified on chromosomes 2, 22 and
Y.
Hide
Molecular
Weight(MW)
92 kDa
Cellular
Localizatio
Cytoplasm, Cytoskeleton, Nucleus
Application
·
WB
Western blot
analysis using CDC27 antibody against HEK293(1) cell lysate.
·
IHC
Immunohistochemical
analysis of paraffin-embedded bladder cancer tissues using CDC27 antibody with
DAB staining.
·
ICC/IF
Immunofluorescence
analysis of CAL-27 cells using CDC27 antibody (green). Blue: DAPI fluorescent
DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555
phalloidin.
Application
Notes
WB:1:200-1:2000
IHC:1:200-1:1000
ICC/IF:1:100-1:500
Additional Information
Form
Liquid
Storage
Instruction
Shipped at 4°C. Store at +4°C short term
(1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer
Purified antibody in PBS with 0.05%
sodium azide.
Product Profile
Product Name
Anti-CDC27 antibody
Antibody Type
Primary Antibodies
Immunogen
Polypeptide
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested
Application
ELISAICC/IFIHCWB
WB:1:200-1:2000
IHC:1:200-1:1000
ICC/IF:1:100-1:500
Species
Reactivity
HumanMouseRat
Concentration
1 mg/ml
Purification
Protein A
Target Information
Gene Symbol
CDC27
Gene Synonyms
APC3
HNUC
NUC2
H-NUC
ANAPC3
CDC27Hs
D0S1430E
D17S978E
Hide
Gene Full Name
cell division cycle 27
Gene Summary
The protein encoded by this gene shares
strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene
product of Schizosaccharomyces pombe nuc 2. This protein is a component of
the anaphase-promoting complex (APC), which is composed of eight protein
subunits and is highly conserved in eukaryotic cells. This complex catalyzes
the formation of cyclin B-ubiquitin conjugate, which is responsible for the
ubiquitin-mediated proteolysis of B-type cyclins. The protein encoded by this
gene and three other members of the APC complex contain tetratricopeptide
(TPR) repeats, which are important for protein-protein interactions. This
protein was shown to interact with mitotic checkpoint proteins including
Mad2, p55CDC and BUBR1, and it may thus be involved in controlling the timing
of mitosis. Alternative splicing of this gene results in multiple transcript
variants. Related pseudogenes have been identified on chromosomes 2, 22 and
Y.
Hide
Molecular
Weight(MW)
92 kDa
Cellular
Localizatio
Cytoplasm, Cytoskeleton, Nucleus
Application
·
WB
Western blot
analysis using CDC27 antibody against HEK293(1) cell lysate.
·
IHC
Immunohistochemical
analysis of paraffin-embedded bladder cancer tissues using CDC27 antibody with
DAB staining.
·
ICC/IF
Immunofluorescence
analysis of CAL-27 cells using CDC27 antibody (green). Blue: DAPI fluorescent
DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555
phalloidin.
Application
Notes
WB:1:200-1:2000
IHC:1:200-1:1000
ICC/IF:1:100-1:500
Additional Information
Form
Liquid
Storage
Instruction
Shipped at 4°C. Store at +4°C short term
(1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer
Purified antibody in PBS with 0.05%
sodium azide.
Product Profile
Product Name
Anti-CDC27 antibody
Antibody Type
Primary Antibodies
Immunogen
Polypeptide
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested
Application
ELISAICC/IFIHCWB
WB:1:200-1:2000
IHC:1:200-1:1000
ICC/IF:1:100-1:500
Species
Reactivity
HumanMouseRat
Concentration
1 mg/ml
Purification
Protein A
Target Information
Gene Symbol
CDC27
Gene Synonyms
APC3
HNUC
NUC2
H-NUC
ANAPC3
CDC27Hs
D0S1430E
D17S978E
Hide
Gene Full Name
cell division cycle 27
Gene Summary
The protein encoded by this gene shares
strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene
product of Schizosaccharomyces pombe nuc 2. This protein is a component of
the anaphase-promoting complex (APC), which is composed of eight protein
subunits and is highly conserved in eukaryotic cells. This complex catalyzes
the formation of cyclin B-ubiquitin conjugate, which is responsible for the
ubiquitin-mediated proteolysis of B-type cyclins. The protein encoded by this
gene and three other members of the APC complex contain tetratricopeptide
(TPR) repeats, which are important for protein-protein interactions. This
protein was shown to interact with mitotic checkpoint proteins including
Mad2, p55CDC and BUBR1, and it may thus be involved in controlling the timing
of mitosis. Alternative splicing of this gene results in multiple transcript
variants. Related pseudogenes have been identified on chromosomes 2, 22 and
Y.
Hide
Molecular
Weight(MW)
92 kDa
Cellular
Localizatio
Cytoplasm, Cytoskeleton, Nucleus
Application
·
WB
Western blot
analysis using CDC27 antibody against HEK293(1) cell lysate.
·
IHC
Immunohistochemical
analysis of paraffin-embedded bladder cancer tissues using CDC27 antibody with
DAB staining.
·
ICC/IF
Immunofluorescence
analysis of CAL-27 cells using CDC27 antibody (green). Blue: DAPI fluorescent
DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555
phalloidin.
Application
Notes
WB:1:200-1:2000
IHC:1:200-1:1000
ICC/IF:1:100-1:500
Additional Information
Form
Liquid
Storage
Instruction
Shipped at 4°C. Store at +4°C short term
(1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer
Purified antibody in PBS with 0.05%
sodium azide.
Product Profile
Product Name
Anti-CDC27 antibody
Antibody Type
Primary Antibodies
Immunogen
Polypeptide
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested
Application
ELISAICC/IFIHCWB
WB:1:200-1:2000
IHC:1:200-1:1000
ICC/IF:1:100-1:500
Species
Reactivity
HumanMouseRat
Concentration
1 mg/ml
Purification
Protein A
Target Information
Gene Symbol
CDC27
Gene Synonyms
APC3
HNUC
NUC2
H-NUC
ANAPC3
CDC27Hs
D0S1430E
D17S978E
Hide
Gene Full Name
cell division cycle 27
Gene Summary
The protein encoded by this gene shares
strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene
product of Schizosaccharomyces pombe nuc 2. This protein is a component of
the anaphase-promoting complex (APC), which is composed of eight protein
subunits and is highly conserved in eukaryotic cells. This complex catalyzes
the formation of cyclin B-ubiquitin conjugate, which is responsible for the
ubiquitin-mediated proteolysis of B-type cyclins. The protein encoded by this
gene and three other members of the APC complex contain tetratricopeptide
(TPR) repeats, which are important for protein-protein interactions. This
protein was shown to interact with mitotic checkpoint proteins including
Mad2, p55CDC and BUBR1, and it may thus be involved in controlling the timing
of mitosis. Alternative splicing of this gene results in multiple transcript
variants. Related pseudogenes have been identified on chromosomes 2, 22 and
Y.
Hide
Molecular
Weight(MW)
92 kDa
Cellular
Localizatio
Cytoplasm, Cytoskeleton, Nucleus
Application
·
WB
Western blot
analysis using CDC27 antibody against HEK293(1) cell lysate.
·
IHC
Immunohistochemical
analysis of paraffin-embedded bladder cancer tissues using CDC27 antibody with
DAB staining.
·
ICC/IF
Immunofluorescence
analysis of CAL-27 cells using CDC27 antibody (green). Blue: DAPI fluorescent
DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555
phalloidin.
Application
Notes
WB:1:200-1:2000
IHC:1:200-1:1000
ICC/IF:1:100-1:500
Additional Information
Form
Liquid
Storage
Instruction
Shipped at 4°C. Store at +4°C short term
(1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer
Purified antibody in PBS with 0.05%
sodium azide.
Product Profile
Product Name
Anti-CDC27 antibody
Antibody Type
Primary Antibodies
Immunogen
Polypeptide
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested
Application
ELISAICC/IFIHCWB
WB:1:200-1:2000
IHC:1:200-1:1000
ICC/IF:1:100-1:500
Species
Reactivity
HumanMouseRat
Concentration
1 mg/ml
Purification
Protein A
Target Information
Gene Symbol
CDC27
Gene Synonyms
APC3
HNUC
NUC2
H-NUC
ANAPC3
CDC27Hs
D0S1430E
D17S978E
Hide
Gene Full Name
cell division cycle 27
Gene Summary
The protein encoded by this gene shares
strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene
product of Schizosaccharomyces pombe nuc 2. This protein is a component of
the anaphase-promoting complex (APC), which is composed of eight protein
subunits and is highly conserved in eukaryotic cells. This complex catalyzes
the formation of cyclin B-ubiquitin conjugate, which is responsible for the
ubiquitin-mediated proteolysis of B-type cyclins. The protein encoded by this
gene and three other members of the APC complex contain tetratricopeptide
(TPR) repeats, which are important for protein-protein interactions. This
protein was shown to interact with mitotic checkpoint proteins including
Mad2, p55CDC and BUBR1, and it may thus be involved in controlling the timing
of mitosis. Alternative splicing of this gene results in multiple transcript
variants. Related pseudogenes have been identified on chromosomes 2, 22 and
Y.
Hide
Molecular
Weight(MW)
92 kDa
Cellular
Localizatio
Cytoplasm, Cytoskeleton, Nucleus
Application
·
WB
Western blot
analysis using CDC27 antibody against HEK293(1) cell lysate.
·
IHC
Immunohistochemical
analysis of paraffin-embedded bladder cancer tissues using CDC27 antibody with
DAB staining.
·
ICC/IF
Immunofluorescence
analysis of CAL-27 cells using CDC27 antibody (green). Blue: DAPI fluorescent
DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555
phalloidin.
Application
Notes
WB:1:200-1:2000
IHC:1:200-1:1000
ICC/IF:1:100-1:500
Additional Information
Form
Liquid
Storage
Instruction
Shipped at 4°C. Store at +4°C short term
(1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer
Purified antibody in PBS with 0.05%
sodium azide.
Product Profile
Product Name
Anti-CDC27 antibody
Antibody Type
Primary Antibodies
Immunogen
Polypeptide
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested
Application
ELISAICC/IFIHCWB
WB:1:200-1:2000
IHC:1:200-1:1000
ICC/IF:1:100-1:500
Species
Reactivity
HumanMouseRat
Concentration
1 mg/ml
Purification
Protein A
Target Information
Gene Symbol
CDC27
Gene Synonyms
APC3
HNUC
NUC2
H-NUC
ANAPC3
CDC27Hs
D0S1430E
D17S978E
Hide
Gene Full Name
cell division cycle 27
Gene Summary
The protein encoded by this gene shares
strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene
product of Schizosaccharomyces pombe nuc 2. This protein is a component of
the anaphase-promoting complex (APC), which is composed of eight protein
subunits and is highly conserved in eukaryotic cells. This complex catalyzes
the formation of cyclin B-ubiquitin conjugate, which is responsible for the
ubiquitin-mediated proteolysis of B-type cyclins. The protein encoded by this
gene and three other members of the APC complex contain tetratricopeptide
(TPR) repeats, which are important for protein-protein interactions. This
protein was shown to interact with mitotic checkpoint proteins including
Mad2, p55CDC and BUBR1, and it may thus be involved in controlling the timing
of mitosis. Alternative splicing of this gene results in multiple transcript
variants. Related pseudogenes have been identified on chromosomes 2, 22 and
Y.
Hide
Molecular
Weight(MW)
92 kDa
Cellular
Localizatio
Cytoplasm, Cytoskeleton, Nucleus
Application
·
WB
Western blot
analysis using CDC27 antibody against HEK293(1) cell lysate.
·
IHC
Immunohistochemical
analysis of paraffin-embedded bladder cancer tissues using CDC27 antibody with
DAB staining.
·
ICC/IF
Immunofluorescence
analysis of CAL-27 cells using CDC27 antibody (green). Blue: DAPI fluorescent
DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555
phalloidin.
Application
Notes
WB:1:200-1:2000
IHC:1:200-1:1000
ICC/IF:1:100-1:500
Additional Information
Form
Liquid
Storage
Instruction
Shipped at 4°C. Store at +4°C short term
(1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer
Purified antibody in PBS with 0.05%
sodium azide.
Product Profile
Product Name
Anti-CDC27 antibody
Antibody Type
Primary Antibodies
Immunogen
Polypeptide
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested
Application
ELISAICC/IFIHCWB
WB:1:200-1:2000
Species
Reactivity
HumanMouseRat
Concentration
1 mg/ml
Purification
Protein A
Target Information
Gene Symbol
CDC27
Gene Synonyms
APC3
Hide
Gene Full Name
cell division cycle 27
Gene Summary
The protein encoded by this gene shares
strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene
product of Schizosaccharomyces pombe nuc 2. This protein is a component of
the anaphase-promoting complex (APC), which is composed of eight protein
subunits and is highly conserved in eukaryotic cells. This complex catalyzes
the formation of cyclin B-ubiquitin conjugate, which is responsible for the
ubiquitin-mediated proteolysis of B-type cyclins. The protein encoded by this
gene and three other members of the APC complex contain tetratricopeptide
(TPR) repeats, which are important for protein-protein interactions. This
protein was shown to interact with mitotic checkpoint proteins including
Mad2, p55CDC and BUBR1, and it may thus be involved in controlling the timing
of mitosis. Alternative splicing of this gene results in multiple transcript
variants. Related pseudogenes have been identified on chromosomes 2, 22 and
Y.
Hide
Molecular
Weight(MW)
92 kDa
Cellular
Localizatio
Cytoplasm, Cytoskeleton, Nucleus
Application
IHC:1:200-1:1000
ICC/IF:1:100-1:500
HNUC
NUC2
H-NUC
ANAPC3
CDC27Hs
D0S1430E
D17S978E
·
WB
Western blot analysis using CDC27 antibody against HEK293(1) cell lysate.
·
IHC
Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using CDC27 antibody with DAB staining.
·
ICC/IF
Immunofluorescence analysis of CAL-27 cells using CDC27 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
|
Application Notes |
WB:1:200-1:2000 |
Additional Information
|
Form |
Liquid |
|
Storage Instruction |
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
|
Storage Buffer |
Purified antibody in PBS with 0.05% sodium azide. |
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