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Product Profile

Product Name	Anti-Connexin-43 antibody
Antibody Type	Primary Antibodies
Immunogen	Polypeptide

Key Feature

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Application	ELISAICC/IFWB
	WB:1:200-1:2000 ICC/IF:1:100-1:500
Species Reactivity	HumanMouseRat
Concentration	1 mg/ml
Purification	Protein A

Target Information

Gene Symbol	GJA1
Gene Synonyms	GJA1 HSS CMDR CX43 EKVP GJAL ODDD AVSD3 EKVP3 HLHS1 PPKCA CXA1 Hide
Gene Full Name	gap junction protein alpha 1
Gene Summary	This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. The encoded protein is the major protein of gap junctions in the heart that are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. A related intronless pseudogene has been mapped to chromosome 5. Mutations in this gene have been associated with oculodentodigital dysplasia, autosomal recessive craniometaphyseal dysplasia and heart malformations. [provided by RefSeq, May 2014] Hide
Molecular Weight(MW)	43 kDa
Cellular Localizatio	Cell membrane. Cell junction > gap junction.

Application

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WB

Western blot analysis using Connexin-43 antibody against HEK293 (1), A549 (2) cell lysate.

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ICC/IF

Immunofluorescence analysis of HEK293 cells using Connexin-43 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Application Notes

WB:1:200-1:2000 ICC/IF:1:100-1:500

Additional Information

Form	Liquid
Storage Instruction	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer	Purified antibody in PBS with 0.05% sodium azide.