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Product Profile

Product Name	Anti-Nanog antibody Catalog Number：OM641807
Antibody Type	Primary Antibodies
Immunogen	Polypeptide

Key Feature

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Application	ELISAICC/IFIHCWB
	WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500
Species Reactivity	HumanMouseRat
Concentration	1 mg/ml
Purification	Protein A

Target Information

Gene Symbol	NANOG
Gene Synonyms	hNanog
Gene Full Name	Nanog homeobox
Gene Summary	The protein encoded by this gene is a DNA binding homeobox transcription factor involved in embryonic stem (ES) cell proliferation, renewal, and pluripotency. The encoded protein can block ES cell differentiation and can also autorepress its own expression in differentiating cells. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015] Hide
Molecular Weight(MW)	35 kDa（Observed:50 kDa）
Source	Rabbit
Cellular Localizatio	Nucleus

Application

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WB

Western blot analysis using Nanog antibody against Hep G2 (1), SK-OV-3 (2) cell lysate.

·

IHC

Immunohistochemical analysis of paraffin-embedded lung cancer tissues using Nanog antibody with DAB staining.

·

ICC/IF

Immunofluorescence analysis of HEK293 cells using Nanog antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Application Notes

WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500

Additional Information

Form	Liquid
Storage Instruction	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer	Purified antibody in PBS with 0.05% sodium azide.