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Product Profile

Product Name	Anti-HSP70 antibody
Antibody Type	Primary Antibodies
Immunogen	Polypeptide

Key Feature

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Application	ELISAICC/IFIHCWB
	WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500
Species Reactivity	HumanMouseRat
Concentration	1 mg/ml
Purification	Protein A

Target Information

Gene Symbol	HSPA1A
Gene Synonyms	HSP72 HSPA1 HSP70I HSP70-1 HSP70-2 HSP70.1 HSP70.2 HSP70-1A HEL-S-103 Hide
Gene Full Name	heat shock protein family A (Hsp70) member 1A
Gene Summary	This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. [provided by RefSeq, Jul 2008] Hide
Molecular Weight(MW)	70 kDa
Source	Rabbit
Cellular Localizatio	Cytoplasm, Cytoskeleton, Nucleus

Application

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WB

Western blot analysis using HSP70 antibody against A431 (1), HEK293 (2), Hep G2 (3), K562 (4) cell lysate.

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IHC

Immunohistochemical analysis of paraffin-embedded lung cancer tissues using HSP70 antibody with DAB staining.

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ICC/IF

Immunofluorescence analysis of NIH/3T3 cells using HSP70 antibody(green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Application Notes

WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500

Additional Information

Form	Liquid
Storage Instruction	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer	Purified antibody in PBS with 0.05% sodium azide.