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Product Profile

Product Name	Anti-WNT3A antibody
Antibody Type	Primary Antibodies
Immunogen	Polypeptide

Key Feature

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Application	ELISAICC/IFIHCWB
	WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500
Species Reactivity	HumanMouseRat
Concentration	1 mg/ml
Purification	Protein A

Target Information

Gene Symbol	WNT3A
Gene Synonyms	WNT-3A
Gene Full Name	Wnt family member 3A
Gene Summary	The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 96% amino acid identity to mouse Wnt3A protein, and 84% to human WNT3 protein, another WNT gene product. This gene is clustered with WNT14 gene, another family member, in chromosome 1q42 region. [provided by RefSeq, Jul 2008] More
Molecular Weight(MW)	39 kDa（Observed:45 kDa）
Source	Rabbit
Cellular Localizatio	Secreted > extracellular space > extracellular matrix.

Application

·          

WB

Western blot analysis using WNT3A antibody against A431 (1) cell lysate.

·          

IHC

Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using WNT3A beta antibody with DAB staining.

·          

ICC/IF

Immunofluorescence analysis of Hela cells using WNT3A antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Application Notes

WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500

Additional Information

Form	Liquid
Storage Instruction	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer	Purified antibody in PBS with 0.05% sodium azi.